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Compatibility PCR cycler & np plates
This compatibility check for PCR cyclers and np PCR plates helps you find the right np PCR plates for your cycler.
Simply select your current cycler to instantly view the compatible np PCR plates. In addition, suitable sealing options for each plate and application are displayed, ensuring you get an optimally matched solution for your workflow.
(Icons zur Vergrößerung berühren. Artikel sind klickbar.)
Die Passgenauigkeiten dieser Tabelle sind Empfehlungen der nerbe plus GmbH & Co. KG.
Die Übertragbarkeit ist daher von Ihnen selbst sehr sorgfältig zu prüfen. Ansprüche aus der Tabelle können nicht abgeleitet werden.
What is PCR and what is a thermocycler used for?
PCR (polymerase chain reaction) is one of the most fundamental and useful methods in modern molecular biology. It is a procedure that allows scientists to multiply specific DNA segments (also known as nucleic acids) from a tiny initial sample (e.g., blood, saliva, or tissue) millions of times. PCR can be thought of as a molecular copying machine.
The role of the thermocycler (PCR cycler)
The thermocycler (or PCR cycler) is the essential device required to perform PCR. The reaction consists of three basic steps, each of which requires precisely defined temperatures:
- Denaturation (approx. 94-98°C): The double-stranded DNA template is separated into two single strands by high heat.
- Annealing (approx. 50-65°C): So-called primers (short pieces of DNA) attach themselves to the single strands at the desired replication start points.
- Elongation (approx. 72°C): The enzyme DNA polymerase begins to synthesize a new complementary DNA strand starting from the primers.
The thermocycler automatically and precisely performs these cycles of rapid temperature changes (thermocycling) dozens of times (typically 25 to 40 cycles). After each cycle, the amount of target DNA doubles exponentially.
Types of devices in the compatibility table
Our compatibility table takes into account different types of devices that use PCR plates, as each type has specific requirements for plate geometry:| Device Type | Function and Application |
|---|---|
| Thermal Cycler | This is the standard PCR device. It serves exclusively to amplify the DNA sample through repeated, precise temperature changes (thermocycling). At the end, it only provides the amplified product, the quantity or success of which must be analyzed separately. |
| Real Time Thermal Cycler | Also known as a qPCR device. It not only performs the amplification but simultaneously measures (in "real time") the amount of DNA being produced via fluorescence signals. This enables quantitative analysis (determination of the initial concentration) and faster, more efficient diagnosis. |
| Sequencer / Genetic Analyzers | These are devices specialized in determining the exact sequence of nucleotides in a DNA strand (sequencing). They often use plate formats for sample preparation or for performing the sequencing reaction itself. The plates must often exhibit extremely high optical purity and dimensional accuracy for this purpose. |
| Scope of Application | Usefulness |
|---|---|
| Medical Diagnostics | Identification of pathogens (viruses, bacteria, e.g., SARS-CoV-2, HIV). Early detection of genetic defects or cancer. |
| Research | Investigation of gene functions, cloning of genes, and creation of DNA sequences for genome research. |
| Forensics | Analysis and comparison of slightest traces of DNA (hair, saliva, blood) to identify individuals at crime scenes. |
| Quality Control | Detection of contaminants in food (e.g., undesirable bacteria) or testing of seeds for genetic modifications. |
np PCR READY
With our pcr-ready products, you don't need to redefine your requirements. Our products give you the certainty that your PCR protocols demand.
- Manufactured in ISO Class 8 cleanrooms or better (DIN EN ISO 14644-1)
- Free of PCR inhibitors
- Free of human DNA
- Free of RNase and DNase
- Free of pyrogens
Frequently Asked Questions about PCR & Thermal Cyclers
What exactly is PCR?
PCR (Polymerase Chain Reaction) is a fundamental method in modern molecular biology. It is a process that allows scientists to amplify specific DNA segments (nucleic acids) millions of times from a tiny initial sample. You can think of PCR as a molecular photocopier.
What is the role of a Thermal Cycler?
The thermal cycler (or PCR cycler) is the essential device required to perform PCR. It automatically and precisely carries out rapid temperature changes (thermocycling) necessary for the three phases of the reaction: Denaturation (separating DNA strands), Annealing (binding of primers), and Elongation (synthesis of new DNA strands).
What is the difference between a standard Thermal Cycler and a Real-Time PCR device?
A standard thermal cycler simply amplifies the DNA for later analysis. A Real-Time Thermal Cycler (qPCR) not only performs amplification but also measures the amount of DNA being formed in "real-time" using fluorescence signals. This allows for immediate quantitative analysis of the sample.
Why is plate geometry so important for Sequencers?
Sequencers and genetic analyzers determine the exact order of nucleotides in a DNA strand. Because these devices are extremely sensitive, the plates used must have superior optical clarity and perfect fitment to ensure accurate data collection.
In which fields is PCR commonly used?
PCR is a powerful tool used in many areas:
- Medical Diagnostics: Identifying pathogens (e.g., viruses like SARS-CoV-2) and early detection of genetic defects.
- Forensics: Analyzing minute DNA traces to identify individuals at crime scenes.
- Quality Control: Detecting contamination in food or testing seeds for genetic modifications.
- Research: Investigating gene functions and genomic mapping.
This compatibility check for PCR cyclers and np PCR plates helps you find the right np PCR plates for your cycler.
Simply select your current cycler to instantly view the compatible np PCR plates. In addition, suitable sealing options for each plate and application are displayed, ensuring you get an optimally matched solution for your workflow.
(Icons zur Vergrößerung berühren. Artikel sind klickbar.)
Die Passgenauigkeiten dieser Tabelle sind Empfehlungen der nerbe plus GmbH & Co. KG.
Die Übertragbarkeit ist daher von Ihnen selbst sehr sorgfältig zu prüfen. Ansprüche aus der Tabelle können nicht abgeleitet werden.
What is PCR and what is a thermocycler used for?
PCR (polymerase chain reaction) is one of the most fundamental and useful methods in modern molecular biology. It is a procedure that allows scientists to multiply specific DNA segments (also known as nucleic acids) from a tiny initial sample (e.g., blood, saliva, or tissue) millions of times. PCR can be thought of as a molecular copying machine.
The role of the thermocycler (PCR cycler)
The thermocycler (or PCR cycler) is the essential device required to perform PCR. The reaction consists of three basic steps, each of which requires precisely defined temperatures:
- Denaturation (approx. 94-98°C): The double-stranded DNA template is separated into two single strands by high heat.
- Annealing (approx. 50-65°C): So-called primers (short pieces of DNA) attach themselves to the single strands at the desired replication start points.
- Elongation (approx. 72°C): The enzyme DNA polymerase begins to synthesize a new complementary DNA strand starting from the primers.
The thermocycler automatically and precisely performs these cycles of rapid temperature changes (thermocycling) dozens of times (typically 25 to 40 cycles). After each cycle, the amount of target DNA doubles exponentially.
Types of devices in the compatibility table
Our compatibility table takes into account different types of devices that use PCR plates, as each type has specific requirements for plate geometry:| Device Type | Function and Application |
|---|---|
| Thermal Cycler | This is the standard PCR device. It serves exclusively to amplify the DNA sample through repeated, precise temperature changes (thermocycling). At the end, it only provides the amplified product, the quantity or success of which must be analyzed separately. |
| Real Time Thermal Cycler | Also known as a qPCR device. It not only performs the amplification but simultaneously measures (in "real time") the amount of DNA being produced via fluorescence signals. This enables quantitative analysis (determination of the initial concentration) and faster, more efficient diagnosis. |
| Sequencer / Genetic Analyzers | These are devices specialized in determining the exact sequence of nucleotides in a DNA strand (sequencing). They often use plate formats for sample preparation or for performing the sequencing reaction itself. The plates must often exhibit extremely high optical purity and dimensional accuracy for this purpose. |
| Scope of Application | Usefulness |
|---|---|
| Medical Diagnostics | Identification of pathogens (viruses, bacteria, e.g., SARS-CoV-2, HIV). Early detection of genetic defects or cancer. |
| Research | Investigation of gene functions, cloning of genes, and creation of DNA sequences for genome research. |
| Forensics | Analysis and comparison of slightest traces of DNA (hair, saliva, blood) to identify individuals at crime scenes. |
| Quality Control | Detection of contaminants in food (e.g., undesirable bacteria) or testing of seeds for genetic modifications. |
np PCR READY
With our pcr-ready products, you don't need to redefine your requirements. Our products give you the certainty that your PCR protocols demand.
- Manufactured in ISO Class 8 cleanrooms or better (DIN EN ISO 14644-1)
- Free of PCR inhibitors
- Free of human DNA
- Free of RNase and DNase
- Free of pyrogens
Frequently Asked Questions about PCR & Thermal Cyclers
What exactly is PCR?
PCR (Polymerase Chain Reaction) is a fundamental method in modern molecular biology. It is a process that allows scientists to amplify specific DNA segments (nucleic acids) millions of times from a tiny initial sample. You can think of PCR as a molecular photocopier.
What is the role of a Thermal Cycler?
The thermal cycler (or PCR cycler) is the essential device required to perform PCR. It automatically and precisely carries out rapid temperature changes (thermocycling) necessary for the three phases of the reaction: Denaturation (separating DNA strands), Annealing (binding of primers), and Elongation (synthesis of new DNA strands).
What is the difference between a standard Thermal Cycler and a Real-Time PCR device?
A standard thermal cycler simply amplifies the DNA for later analysis. A Real-Time Thermal Cycler (qPCR) not only performs amplification but also measures the amount of DNA being formed in "real-time" using fluorescence signals. This allows for immediate quantitative analysis of the sample.
Why is plate geometry so important for Sequencers?
Sequencers and genetic analyzers determine the exact order of nucleotides in a DNA strand. Because these devices are extremely sensitive, the plates used must have superior optical clarity and perfect fitment to ensure accurate data collection.
In which fields is PCR commonly used?
PCR is a powerful tool used in many areas:
- Medical Diagnostics: Identifying pathogens (e.g., viruses like SARS-CoV-2) and early detection of genetic defects.
- Forensics: Analyzing minute DNA traces to identify individuals at crime scenes.
- Quality Control: Detecting contamination in food or testing seeds for genetic modifications.
- Research: Investigating gene functions and genomic mapping.